Whitepapers

Superior resolution in size exclusion chromatography (SEC) protein separation made possible by the Agilent 1290 Infinity II Bio LC System.

The Agilent 1290 Infinity II Bio LC System is the new platform for UV and MS-based primary structure and PTMs analysis of mAbs.

This study demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent AssayMAP Bravo liquid handling platform.

Peptide-mapping analysis shows excellent performance and high method compatibility compared to the Agilent 1290 Infinity II LC System.

Here, we demonstrate how you can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA with our SpectraMax® Multi-Mode Microplate Readers and the Quant-iT Pico Green assay.

Here, Sartorius provides an overview of cell line development and the associated hurdles. We will then discuss potential solutions that form part of an effective risk-management strategy and improve productivity.

This document outlines references to 21 CFR Part 11 and EudraLex Annex 11 and how they apply to the implementation of SoftMax Pro GxP Data Acquisition and Analysis Software in regulated environments.

Combining high performance protein A capturing and a single polishing step on a salt-tolerant anion exchange resin, downstream costs were reduced by 45% in this Tosoh 2-step process for mAb purification.

Small batch applications may benefit from pre-sterilized ready-to-use primary packaging components supplied directly to the filling station as an alternative to operating a washing and sterilizing line. 

Dynamic and static light scattering are applied to test the effect of ramp rate on thermostability measurements of an IgG. The choice of heating rate significantly impacts results.

This application demonstrates excellent performance in method development for the analysis of raw materials for oligonucleotide synthesis using a biocompatible UHPLC.

The coupling of FPLC to MALS not only detects but also quantitatively determines the absolute molecular weight and size of each species, including the targeted molecules, impurities, and its aggregates.

Comparison of HPLC and Octet® system to determine MAb concentration in a complex feedstock using mAbs breakthrough as the analyte while monitoring method accuracy, precision, dynamic range, LoQ and cost per sample.

Pharmacopoeias are collections of recognized and binding quality regulations that specify, among other things, test requirements and methods for the analysis of drugs. Both the European Pharmacopoeia (Ph. Eur.) and the United States Pharmacopeia (USP) contain specific chapters that deal with the use of laboratory balances. The chapters emphasize the criticality of weighing samples accurately in pharmaceutical analytical processes. The aim of this white paper is to present the testing requirements for laboratory balances as described in the relevant chapters of the pharmacopoeias and the associated certificates offered by Sartorius Service.

The purity of the solvents used, primarily that of water, is a decisive criterion for interference-free and reproducible analysis by liquid chromatography and for the sensitivity of this method, especially for applications in trace analysis. In a study, different sources of ultrapure water used as eluents were compared in high-performance liquid chromatography with diode-array detectors (HPLC-DAD) and mass spectrometry (MS) systems in various experiments