Sartorius

Articles

Host Cell Protein Detection, Precise and Sensitive

A completely hands-off, walk-away HCP assay analyzing 96 samples can be set up to run automatically on an Octet® RH96 instrument with results obtained in one hour.

New Octet® Bioprocessing Applications Page

To accelerate commercialization of biotherapeutics, organizations need to optimize processes by integrating advanced analytical tools that maximize quality, safety, and efficacy. Octet® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity, and antibody/protein quantitation. Based on Bio-Layer Interferometry (BLI), the systems enable an enviable variety of applications throughout biologics development, from early selection to validation and manufacturing. Read on to learn about the implementation in the production of biotherapeutics for titer, host cell protein analysis, residual protein detection, sialic acid content detection, potency assay development, Fab activity assay development, and more.

Residual Protein A Detection, Easier and Faster Than ELISA

A more streamlined technique with reduced hands-on time using the Octet® platform. For customers who require a sensitive and robust assay for measuring small amounts of leached Protein A in antibody or Fc-fusion protein samples.

Whitepaper: Titer and Protein Quantitation in as Little as Two Minutes

Replace ELISA and HPLC with Octet® systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays are also easy to develop and transfer to QC and manufacturing. In this whitepaper you will learn about analyzing a full plate (96-samples) of IgG titer in as little as two minutes, assay directly in crude and unpurified samples, and automation for extending un-attended run times and high throughput analysis.

Shedding Light on the Dark Art of Bioprocess Scaling

In this White Paper, Sartorius outlines the challenges of scaling a bioprocess and presents solutions to maintain control over critical quality attributes.

Fast and Accurate Titer Determination

Replace ELISA and HPLC with Octet® systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays are also easy to develop and transfer to QC and manufacturing.

A Guide to Accelerating Cell Line Development for Commercial Production v2

Here, Sartorius provides an overview of cell line development and the associated hurdles. We will then discuss potential solutions that form part of an effective risk-management strategy and improve productivity.

MAb Quantitation: Protein A HPLC vs. Protein A Bio-Layer Interferometry

Comparison of HPLC and Octet® system to determine MAb concentration in a complex feedstock using mAbs breakthrough as the analyte while monitoring method accuracy, precision, dynamic range, LoQ and cost per sample.

Use of laboratory balances in the pharmaceutical industry

Pharmacopoeias are collections of recognized and binding quality regulations that specify, among other things, test requirements and methods for the analysis of drugs. Both the European Pharmacopoeia (Ph. Eur.) and the United States Pharmacopeia (USP) contain specific chapters that deal with the use of laboratory balances. The chapters emphasize the criticality of weighing samples accurately in pharmaceutical analytical processes. The aim of this white paper is to present the testing requirements for laboratory balances as described in the relevant chapters of the pharmacopoeias and the associated certificates offered by Sartorius Service.

Arium® Ultrapure Water for Trace Analysis

The purity of the solvents used, primarily that of water, is a decisive criterion for interference-free and reproducible analysis by liquid chromatography and for the sensitivity of this method, especially for applications in trace analysis. In a study, different sources of ultrapure water used as eluents were compared in high-performance liquid chromatography with diode-array detectors (HPLC-DAD) and mass spectrometry (MS) systems in various experiments