Sartorius

Articles

Rapid AAV Titer for Bioprocessing in Gene Therapy Using Octet® BLI Platform

Adeno-associated virus (AAV) is a widely used viral vector for the delivery of genetic material in gene therapy. Vector development and production at an industrial scale requires an efficient method for accurate quantification of viral capsids. Unfortunately, traditional techniques such as ELISA, HPLC, and ddPCR are time consuming and labor-intensive. This podcast introduces a rapid, label-free method for the direct capture and quantitation of various AAV serotypes using the Octet® Bio-Layer Interferometry (BLI) platform with Octet® AAVX Biosensors. These biosensors deliver results in as little as 15 minutes and can be used to quantitate AAV capsids in both crude and purified samples.

Host Cell Protein Detection, Precise and Sensitive

A completely hands-off, walk-away HCP assay analyzing 96 samples can be set up to run automatically on an Octet® RH96 instrument with results obtained in one hour.

New Octet® Bioprocessing Applications Page

To accelerate commercialization of biotherapeutics, organizations need to optimize processes by integrating advanced analytical tools that maximize quality, safety, and efficacy. Octet® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity, and antibody/protein quantitation. Based on Bio-Layer Interferometry (BLI), the systems enable an enviable variety of applications throughout biologics development, from early selection to validation and manufacturing. Read on to learn about the implementation in the production of biotherapeutics for titer, host cell protein analysis, residual protein detection, sialic acid content detection, potency assay development, Fab activity assay development, and more.

Residual Protein A Detection, Easier and Faster Than ELISA

A more streamlined technique with reduced hands-on time using the Octet® platform. For customers who require a sensitive and robust assay for measuring small amounts of leached Protein A in antibody or Fc-fusion protein samples.

Whitepaper: Titer and Protein Quantitation in as Little as Two Minutes

Replace ELISA and HPLC with Octet® systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays are also easy to develop and transfer to QC and manufacturing. In this whitepaper you will learn about analyzing a full plate (96-samples) of IgG titer in as little as two minutes, assay directly in crude and unpurified samples, and automation for extending un-attended run times and high throughput analysis.

Shedding Light on the Dark Art of Bioprocess Scaling

In this White Paper, Sartorius outlines the challenges of scaling a bioprocess and presents solutions to maintain control over critical quality attributes.

Fast and Accurate Titer Determination

Replace ELISA and HPLC with Octet® systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays are also easy to develop and transfer to QC and manufacturing.

A Guide to Accelerating Cell Line Development for Commercial Production v2

Here, Sartorius provides an overview of cell line development and the associated hurdles. We will then discuss potential solutions that form part of an effective risk-management strategy and improve productivity.

MAb Quantitation: Protein A HPLC vs. Protein A Bio-Layer Interferometry

Comparison of HPLC and Octet® system to determine MAb concentration in a complex feedstock using mAbs breakthrough as the analyte while monitoring method accuracy, precision, dynamic range, LoQ and cost per sample.