Eppendorf

Articles by Eppendorf

Novel fields, such as cell and gene therapy require large amounts of cells to be produced and are therefore dependent on efficient scale‐up, the process of increasing bioprocess dimensions. However, as multiple parameters are influenced during this process, it is not easy to keep yields consistent between small‐ and large‐scale approaches. Therefore, in order to design the scale‐up process as efficient as possible, different strategies focusing on different parameters were established over time. In this ebook, Eppendorf thought leaders will give an overview of the current gold standards in scale‐up and share their opinions on advantages and disadvantages of different strategies. Furthermore, scale‐up of both bacterial and mammalian cell culture is experimentally demonstrated in this publication.

Bioreactors have become an industry backbone for growing vast amounts of cells to produce therapeutic agents, as the process involves a precise balance between nutrient availability, temperature control and oxygen/carbon dioxide balance, among other parameters which all must be closely monitored. Learn in this ebook about recent advances in bioprocessing for biologics and gene therapy vectors.

Whether you are new or experienced in bioprocessing, this website arms you with useful knowledge from general bioprocessing topics to expert advice.

This ebook gives an overview of the key characteristics of a stirred-tank bioreactor, its different operation modes, as well as the possibilities of scale-up and tech transfer.

You would like to cultivate your cells in a bioreactor but are unsure how to get started? We guide you through a complete bioprocess from inoculum preparation to sample analysis.

In the biopharmaceutical industry, upstream processing for plasmid DNA production is usually optimized at small scale. We demonstrate the feasibility of using the DASbox® Mini Bioreactor System for process development.