Eppendorf

In this eBook, Eppendorf experts will give an overview of the most used vaccine platforms and discuss the important role of stirred-tank bioreactors in this life-saving field.

This application note demonstrates the feasibility of an efficient and scalable AAV production platform, using a suspension-adapted HEK293 cell line (Expi293F) as the host.

In this webinar, Eppendorf experts will give an overview of the most used vaccine platforms and discuss the important role of stirred-tank bioreactors in this life-saving field.

Novel fields, such as cell and gene therapy require large amounts of cells to be produced and are therefore dependent on efficient scale‐up, the process of increasing bioprocess dimensions. However, as multiple parameters are influenced during this process, it is not easy to keep yields consistent between small‐ and large‐scale approaches. Therefore, in order to design the scale‐up process as efficient as possible, different strategies focusing on different parameters were established over time. In this ebook, Eppendorf thought leaders will give an overview of the current gold standards in scale‐up and share their opinions on advantages and disadvantages of different strategies. Furthermore, scale‐up of both bacterial and mammalian cell culture is experimentally demonstrated in this publication.

Bioreactors have become an industry backbone for growing vast amounts of cells to produce therapeutic agents, as the process involves a precise balance between nutrient availability, temperature control and oxygen/carbon dioxide balance, among other parameters which all must be closely monitored. Learn in this ebook about recent advances in bioprocessing for biologics and gene therapy vectors.

This application note provides an in-depth look at the batch, fed-batch, and perfusion bioreactor operation modes using the commonly employed CHO cell line as an example.

Whether you are new or experienced in bioprocessing, this website arms you with useful knowledge from general bioprocessing topics to expert advice.

This white paper addresses the distinct steps in the plasmid production workflow and provides advice on microbial process optimization to increase yields

This ebook gives an overview of the key characteristics of a stirred-tank bioreactor, its different operation modes, as well as the possibilities of scale-up and tech transfer.

This ebook discusses how bioreactors and their components can be employed in cell culture with the goal of increasing process reproducibility.

Webinar Date/Time: Tue, Jun 18, 2024 10:00 AM EDT | 4:00 PM CEST

This application note examines the suitability of BioBLU® 0.3c Single-Use Bioreactors and the DASbox Mini Bioreactor System in the long-term expansion of CD4+ T cells.

You would like to cultivate your cells in a bioreactor but are unsure how to get started? We guide you through a complete bioprocess from inoculum preparation to sample analysis.

We describe the setup of a small scale perfusion bioprocess using a DASbox® Mini Bioreactor System. The system was suitable for the prolonged cultivation of CHO and HEK293 cells.

We tested the suitability of DASbox® Mini Bioreactor System in the long-term expansion of CD4+ T cells as well as the impact of different oxygen tensions on cell proliferation.

In the biopharmaceutical industry, upstream processing for plasmid DNA production is usually optimized at small scale. We demonstrate the feasibility of using the DASbox® Mini Bioreactor System for process development.