This application note examines the suitability of BioBLU® 0.3c Single-Use Bioreactors and the DASbox Mini Bioreactor System in the long-term expansion of CD4+ T cells.
You would like to cultivate your cells in a bioreactor but are unsure how to get started? We guide you through a complete bioprocess from inoculum preparation to sample analysis.
We describe the setup of a small scale perfusion bioprocess using a DASbox® Mini Bioreactor System. The system was suitable for the prolonged cultivation of CHO and HEK293 cells.
We tested the suitability of DASbox® Mini Bioreactor System in the long-term expansion of CD4+ T cells as well as the impact of different oxygen tensions on cell proliferation.
In the biopharmaceutical industry, upstream processing for plasmid DNA production is usually optimized at small scale. We demonstrate the feasibility of using the DASbox® Mini Bioreactor System for process development.