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Articles

Robust Oligonucleotide Molecular Weight Confirmation by LC-MS

How to Achieve Confident Oligonucleotide Characterization and Purification

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Discover how to accurately analyze purity and impurities, determine sequences, and more with next-generation technologies from Agilent.

Webcasts

Experiencing Bottlenecks in Your Raw Material ID?

Webinar Date/Time: Tuesday, January 9, 2024 at 11am EST | 8am PST | 4pm GMT| 5pm CET

Upgrading to Zero-Waste Raw Material Identification with the Agilent Vaya

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Reaching sustainability and efficiency goals simultaneously is possible in RMID, a regulatory-enforced part of (bio)pharmaceutical drug manufacturing.

Best Practices for Analysis of In Vitro Transcribed (IVT) mRNA Using the Agilent Fragment Analyzer systems

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This technical overview discusses best handling practices for IVT mRNA and analysis with the Fragment Analyzer systems, including sample handling and quantification tips.

Webcasts

Boost Productivity and Eliminate Waste in Raw Material ID

Webinar Date/Time: Thu, Aug 17, 2023 11:00 AM EDT

Oligo Workflow Resource Guide

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End-to-End Workflow Solutions for Oligonucleotide Analysis - From research discovery to production QA/QC

End-to-End Oligonucleotide Solutions

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This short video provides an overview of a complete, end-to-end oligonucleotide workflow solutions

MS1 Oligonucleotide Characterization Using LC/Q-TOF with HILIC Chromatography

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In this application note, LC separation and MS1 mass identification of a variety of oligos without the use of ion‑pairing reagents is demonstrated. The LC separation allows subsequent positive mode use with little to no flushing or hardware changes. This HILIC-based method uses an Agilent InfintyLab Poroshell 120 HILIC-Z column and MS-friendly ammonium acetate-based mobile phases. The samples were analyzed on an Agilent 1290 Infinity II LC system and a 6545XT AdvanceBio quadrupole time-of-flight mass spectrometer (LC/Q-TOF).

Oligonucleotide Characterization by Agilent 1290 Infinity II Bio LC and 6545XT AdvanceBio LC/Q-TOF

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Characterization of oligonucleotides requires robust analytical instrumentation and methods as well as ease-of-use data analysis tools. Biocompatibility mitigates non-specific sample binding to flow path and it ensures the integrity of biomolecules and robustness of the system. In this study, two workflows, the Target Plus Impurities (TPI) and Sequence Confirmation workflows in Agilent MassHunter BioConfirm software, were carried out to characterize two oligonucleotide samples.