Field-flow fractionation with multi-angle and dynamic light scattering (FFF-MALS-DLS) combines size-based separation with absolute size and structure determination.
SEC-MALS characterizes PEGylated proteins to determine the protein-PEG ratio and the molar masses of the constituent protein and PEG at each eluting fraction.
Wyatt's instruments and techniques constitute platform methods for effective product and process development, as well as quality control, of AAVs for gene therapy.
Multi-angle light scattering is a powerful technology for the biophysical characterization of biologics and LNPs. It can also serve as an effective PAT tool to monitor product attributes during DSP.
This note demonstrates the use of DLS and online multi-angle light scattering with field-flow fractionation (FFF-MALS) for characterizing retroviral viral vectors.
SEC-MALS and CG-MALS characterize viral glycoprotein (vGP) self-association and the absolute stoichiometry and affinity of vGP: antibody interactions.
Light scattering quantifies key attributes of lipid nanoparticle gene vectors, such as payload, size, titer, and stability in formulation and physiological media.
Light scattering facilitates vaccine development by enabling the analysis of size, shape, molar mass, and other parameters derived from these properties.
Many biologics must undergo freeze-thaw stability testing during product development. We describe a method for performing this test using a DLS plate reader.
SEC-MALS quantifies biophysical attributes of mRNA, including molar mass, percent aggregates, Rg and Rh, and how they are affected by formulation and storage conditions.