Optimizing Recombinant Microbial Fermentation Processes: An Integrated Approach

July 15, 2002
Karl Bayer

,
Monika Cserjan-Puschmann

,
Reingard Grabherr

,
Franz Clementschitsch

,
Gerald Striedner

BioPharm International, BioPharm International-07-01-2002, Volume 15, Issue 7

by Karl Bayer, Monika Cserjan-Puschmann, Reingard Grabherr, Gerald Striedner, and Franz Clementschitsch, Institute of Applied Microbiology, Vienna, Austria A new strategy for controlling recombinant gene expression improves efficiency, maximizes host vector exploitation, reduces costs, improves product consistency, and accelerates product development. Continuous feeds of limited amounts of inducer proportional to biomass growth grant optimal control over the ratio between gene expression and host cell metabolism, providing stable, prolonged recombinant protein production.