Executive Summary: Therapeutic Nucleic Acids: Advanced Solutions for Downstream Processing and Analysis

Advanced chromatographic and analytical approaches can help overcome the complex downstream purification challenges of therapeutic nucleic acids; these innovations include on-column DMT cleavage and real-time anion-exchange chromatography coupled with multi-angle light scattering (AEX MALS) integration. By exploring next-generation resin technologies and sustainable, solvent-free workflows, this article highlights how process scientists can enhance purity, yield, and scalability for complex drug manufacturing.