Designs for Bioassays with Plate Location Effects

Meena Subramanyam;Brian Schlain;Hitendra S. Jethwa;Michael Molloy;Kevin Moulder;Bhavna Bhatt;

Publication

Article

November 15, 2001

BioPharm International

BioPharm International-11-01-2001

Volume14

Issue 11

Designs for Bioassays with Plate Location Effects

Author(s):

Meena Subramanyam,Brian Schlain

by Brian Schlain, Hitendra S. Jethwa, Meena Subramanyam, Kevin Moulder, Bhavna Bhatt, and Michael Molloy Standard 96-well microplate assays are prone to edge and bowl effects. Such plate location effects can badly bias potency estimates. Easy-to-use plate layouts enable scientists to adjust estimates for such effects when analyzing new production drug batches. This article is indebted to the work reported by Russell Reeve (BioPharm, July 2000) and the subsequent letters from David Lansky and Reeve (BioPharm, September 2000).

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Articles in this issue

Business Matters: Eclipsed by Catastrophe

Systematic Troubleshooting for LC/MS/MS: Part 1, Sample Preparation and Chromatography

Designs for Bioassays with Plate Location Effects

Liposomes (a Review), Part One: Manufacturing Issues

Simplifying Pharmacologic Analysis with Programmable Scientific Software

Outsourcing Outlook: Truth in Outsourcing

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