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Controlling certain atmospheric conditions in a closed NGI processing chamber can reduce bioburden without disinfectants.
Chemical disinfectants pose risks to live cells. In a closed cell handling system, temperature and relative humidity (RH) were examined as a means to control microbes. Compared with biological safety cabinet conditions, lower RH and physiologic temperatures increased log reductions on polypropylene and steel surfaces. Environmental control can reduce the need for disinfectants.
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Submitted: December 2, 2020
Accepted: February 5, 2021
Yan He is the senior scientist; Shannon Darou is a technician; Stassa P.D. Henn is an intern; Rose Walter is an intern; Randy Yerden is the chief executive officer; and Alicia D. Henn*, firstname.lastname@example.org, is the chief scientific officer, all at BioSpherix, Ltd., 25 union St. Parish, NY; and Tony Cundell is a consultant of BioSpherix, Ltd., and located at Microbiological Consulting, LLC, Scarsdale, NY.
Vol. 34, No. 6
When referring to this article, please cite it as Y. He et al., “Temperature and Relative Humidity Control to Reduce Bioburden in a Closed Cell Processing and Production System without Disinfectants,” BioPharm International 34 (6) 2021.