3D iPSC Expansion as Aggregates within a Distek BIOne Single-Use Bioreactor with a Microsparger Used for Media Exchange

Discover how scalable 3D suspension culture can improve human induced pluripotent stem cell (iPSC) expansion compared to traditional 2D methods. This poster demonstrates process development within a BIOne 2L single-use bioreactor, highlighting aggregate size control, cell viability, expansion performance, and cost efficiency while achieving a 60-fold expansion over five days using Defined Bioscience HiDef® S8™ medium.