Miladys Limonta Fernández

Havana

Cuba

Principal Researcher at the Centre for Genetic Engineering and Biotechnology

Articles

Scale up of Fed-Batch Culture to Produce Plasmid DNA in Escherichia coli (Peer Reviewed)

The authors present scale-up from a 5-L fermentor to a 50-L pilot-scale using the criterion of constant power consumption per unit liquid volume.

The Purification of Plasmid DNA for Clinical Trials Using Membrane Chromatography

Membrane chromatography ensures purity at high flow rates.

High-Cell-Density Culture to Produce Plasmid DNA for Gene Therapy in E. coli

How to produce Plasmid DNA in a high-cell-density culture.

Plasmid DNA Recovery Using Size-Exclusion and Perfusion Chromatography

Scalable method to recover plasmid-DNA.