Precipitation prior to capture chromatography offers a simple, robust, and economical method to remove CHO host cell proteins and DNA.
ACF medium sped up cell-line development.
In three non-affinity purification processes based on cation exchange capture with high binding capacity, applying a host cell protein exclusion strategy enabled robust scale up and better economics.
An alternative approach to traditional Protein A schemes is comparable in overall efficiency, product recovery, and quality.