Agilent

Articles by Agilent

Methodology Development for Complete Nucleoside and Nucleotide Monitoring within Cell Samples

Tuesday, February 8th, 2022 at 9am EST | 3pm CET | 2pm GMT and 2pm EST | 11am PST | 7pm GMT Approaches and lessons learned on developing an HPLC methodology to cover a complex and broad range of polar analytes: the complete cellular pool of nucleosides and nucleotides plus our drug candidates and associated prodrugs. The utility of PEEK-lined columns and newer HILIC-Z column phases is highlighted in this anecdote of HPLC methodology development.

Process Analytical Technology (PAT): Online Monitoring and Control of Upstream Titer, Product Quality and Amino Acid Content Using 2D-LC with SegFlow Interface-Use Material Selection for Critical Storage Operations in Gene Therapy Development and Scale-up

*Europe Broadcast: Tuesday, May 18, 2021 9am EDT | 2pm BST | 3pm CEST and US Broadcast: Tuesday, May 18, 2021 2pm EDT | 1pm CDT | 11am PDT* Biopharmaceutical companies are transitioning from “quality-by-testing” philosophy to “quality-by-design” paradigms, and establishing QbD-based PAT tools to increase product and process understanding. Due to the deficit of analytical methods to support QbD-driven continuous bioprocessing, biopharmaceutical companies are actively developing PAT tools to comply with QbD regulations. *On Demand Until May 18, 2022*