A Simple Step-by-Step Protocol for Hydrophilic Interaction Liquid Chromatography (HILIC) Development

Key Takeaways

• A rationally designed protocol for HILIC method development focuses on optimizing stationary phases and mobile phase composition for improved separation of polar compounds.
• Key parameters such as pH and buffer concentration are adjusted to enhance reproducibility and reduce development time in HILIC methods.
• The protocol provides a robust framework for efficient chromatographic separations, particularly beneficial for complex sample matrices in analytical applications.