
News|Videos|November 5, 2025
A Step-by-Step Approach for Method Development to Generate a Successful HILIC Separation
Key Takeaways
- HILIC is effective for separating polar compounds, offering enhanced resolution and selectivity compared to reversed-phase chromatography.
- The method development process is crucial for optimizing HILIC parameters, ensuring effective separation of target analytes.
This video explains Hydrophilic Interaction Liquid Chromatography (HILIC) and discusses its key advantages. The video also discusses the importance of resolution and selectivity, and walks through a simple method development approach, using caffeine as an example.
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