BioPharm International-07-15-2003

Guide to Columns

July 15, 2003

Column chromatography: Separation method in which different components of a mixture migrate down through a column at different rates of speed based on their various chemical properties.

Guide to Columns

July 15, 2003

Glossary

An overview of trends in process chromatography; how new innovations have improved production; and a guide to manufacturers involved in selecting chromatography equipment.

For maximum efficiency, high product yield, and purity, you must produce a homogeneous packed bed every time you perform a separation. Irregularities in packing cause uneven flow within the bed, resulting in band broadening, zone mixing, changes in flow rate, and subsequent loss of product yield and quality. Here we provide guidelines for reproducible column packing along with useful troubleshooting tips.