Column Packing for Process-Scale Chromatography: Guidelines for Reproducibility

Published on: 
BioPharm International, BioPharm International-07-15-2003, Volume 2003 Supplement, Issue 2

For maximum efficiency, high product yield, and purity, you must produce a homogeneous packed bed every time you perform a separation. Irregularities in packing cause uneven flow within the bed, resulting in band broadening, zone mixing, changes in flow rate, and subsequent loss of product yield and quality. Here we provide guidelines for reproducible column packing along with useful troubleshooting tips.