Excipients in Biopharma: Polysorbate 20 and 80 Analysis

Polysorbate 20 and 80 protect biologic formulations, but their complex composition makes them hard to measure. Learn practical LC-based approaches for detecting and quantifying polysorbates in protein drug products.

Register Free: https://www.chromatographyonline.com/lcgc_w/polysorbate

Event Overview:

Polysorbate 20 and polysorbate 80 are among the most widely used excipients in protein-based biopharmaceuticals, helping stabilize formulations and protect against aggregation. Their heterogeneous composition, lack of a strong UV chromophore, and susceptibility to degradation make accurate detection and quantification a persistent analytical challenge. In this webcast, scientists share practical liquid chromatography approaches, including charged aerosol detection and mass detection, for characterizing and quantifying polysorbates in raw materials and drug products. Attendees will leave with actionable strategies to streamline method development and support formulation development and quality control.

Key Learning Objectives:

• Understand why polysorbate analysis is challenging in biologic formulations.
• Learn how charged aerosol detection enables polysorbate quantification.
• Apply LC-based workflows to monitor polysorbates in development and QC.

Who Should Attend:

• Analytical development and formulation scientists in biopharma
• QC and CMC scientists working with protein therapeutics
• Lab managers and directors in biologics development

Speakers:

Margaret Maziarz, MS
Principal Scientist
Waters Corporation

Margaret Maziarz, MS, is a principal scientist at Waters Corporation in Milford, Massachusetts, responsible for the development, validation, modernization, scaling, and transfer of analytical methods. She specializes in new application development and creates technical seminars, webinars, and demo projects that support teams globally. Before joining Waters in 2010, she held various scientific positions in the life sciences industry.

Pawel Bigos, BS
Senior Scientist
Waters Corporation

Pawel Bigos, BS, is a senior scientist in the LC-MS Biologics group at Waters Corporation in Milford, Massachusetts. He holds a BS in chemistry from the University of South Florida and previously worked at Frequency Therapeutics, Novartis, and Symbiotic Research. Since joining Waters in 2022, he has focused on biomolecule separations and supports the development, commercialization, and application of new products.

Register Free: https://www.chromatographyonline.com/lcgc_w/polysorbate