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February 1, 2021
Agilent Technologies

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Analysis of Camelid Single-Domain Antibodies Using Agilent AdvanceBio SEC 120 Å 1.9 µm and AdvanceBio HIC Columns
Achieve high-resolution nanobody aggregate and fragment characterization with sub-2µm SEC UV and native LC/MS detection, and resolve important post translational modifications with non-denaturing HIC.

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Mass Spectrometric Characterization of Antibody-siRNA Conjugates using the Agilent 6545XT AdvanceBio LC/Q-TOF
Denaturing (reversed-phase) and non-denaturing (size exclusion) analyses of novel siRNA conjugated monoclonal antibodies by LC/MS

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Elevate Your mAb Aggregate Analysis: High-resolution SEC with the Agilent 1290 Infinity II Bio LC System
How to optimize your ultra-high performance size exclusion separations, using biocompatible UHPLC instrumentation with attention to minimizing extra-column dead volume.

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An Improved Workflow for Profiling and Quantitation of Sialic Acids in Biotherapeutics
This work highlights a newly-released Agilent workflow for profiling and quantitation of sialic acids in biotherapeutic glycoproteins. This new workflow includes all the reagents necessary for sample preparation of sialic acids and also features a qualitative Sialic Acid Reference Panel ase well as quantitative NANA and NGNA standards.

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Analysis of a Synthetic Peptide and Its Impurities Using mass spectrometry compatible mobile phases with an Agilent AdvanceBio Peptide Plus column
The benefits of using a charged-surface modified C18 reversed-phase column for improved performance and peak shape of a synthetic peptide using LC/MS methodology.

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Seamless Method Transfer to the Agilent 1290 Infinity II Bio LC System Peptide-mapping analysis shows excellent performance and high method compatibility compared to the Agilent 1290 Infinity II LC System
Transitioning validated methods between instruments is sometimes a challenge. Using a peptide mapping example, we demonstrate exceptional data consistency between the Agilent 1290 Infinity II LC and the biocompatible Agilent 1290 Infinity II Bio LC.

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How Shallow Can You Go? Refining charge variant analysis of mAbs with the Agilent 1290 Infinity II Bio LC System
Excellent rentention time and peak area reproducibility are possible even with highly challenging shallow gradients for ion exchange chromatography.

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Improved Lifetime of Bio-Monolith Protein A Columns for Titer Determination
Learn how to increase column lifetime with an improved monolithic affinity chromatography column designed for rapid screening of monoclonal antibody titer in cell culture supernatant.

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High Salt—High Reproducibility Analysis of antibody drug conjugates using hydrophobic interaction chromatography with the Agilent 1290 Infinity II Bio LC System
By removing iron from the LC flow path, the Agilent Infinity II Bio LC is perfectly suited for the high salt concentrations used in HIC-based drug-to-antibody ratio characterization.

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Isolate and Reanalyze Pharma Impurities with the Agilent 1290 Infinity II Autoscale Preparative LC/MSD System
Learn about Agilent's Gly-X N-glycan sample preparation platform and available dye options for LC/FLD and LC/MS analysis of N-glycans from biotherapeutic glycoproteins.

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