Validation of Quantitative PCR Assays?Addressing Virus Contamination Concerns

Archie Lovatt, PhD

Scientific Director at BioReliance UK

Iain Doherty

Donna McMutrie

John Black

BioPharm International, BioPharm International-03-15-2002, Volume 15, Issue 3

by Archie Lovatt, Donna McMutrie, John Black, and Iain Doherty Q-One Biotech Ltd. Real-time quantitative PCR has revolutionized the detection and quantification of viral contaminants. Quantitative PCR assays can detect noncytopathic viruses that have recently become a concern in testing for biopharmaceutical safety. High versatility reproducibility, and throughput of such assays decrease the time spent in development, testing, and regulatory compliance.