Oligonucleotide Purification and Characterization - Tips for Success

Webcast

Wednesday, March 30th, 2022 at 11am SST | 2pm AEDT | 10am ICT | 12pm KST Applying the appropriate tools for oligonucleotide purification and subsequent analysis is a unique challenge requiring application specific solutions. Join Drs. Anne Blackwell and Matthew L. Turner of Agilent Technologies for tips on oligonucleotide analysis and purification with Agilent’s suite of Bio LC solutions.

Register Free: https://www.biopharminternational.com/bp_w/glycomics

Event Overview:

With the enormous potential of oligonucleotide therapeutics, there is more interest than ever in choosing the best tools for oligonucleotide synthesis, analysis, and purification.

Given the complex impurity profiles, varying sequence modifications, and lengths, thoughtful optimization of HPLC methodologies for characterization and purification is essential to obtain pure oligonucleotides for research, diagnostics, and therapeutics. This webinar will discuss the common challenges associated with oligonucleotide analysis and purification, focusing on Agilent’s suite of Bio LC column solutions designed to address the complex needs of the market.

The following will be covered:

  • Technical challenges and impurities associated with oligonucleotide synthesis
  • Oligonucleotide analysis with varying oligonucleotide structures (size, modifications)
  • Anion exchange and ion-pair reverse phase, what to choose based on purity, scale, temperature, and instrumentation
  • Method optimization considerations
  • Scaling up your purification

Key Learning Objectives:

  • Technical challenges and impurities associated with oligonucleotide synthesis
  • Oligonucleotide analysis with varying oligonucleotide structures (size, modifications)
  • AEX vs IP-RP and the best choice based on purity, scale, temperature, and instrumentation
  • Method optimization considerations
  • Scaling up your purification

Who Should Attend:

  • QA/QC laboratories
  • Biopharma analytical development scientists
  • Biopharma process development scientists
  • CDMO labs
  • Diagnostic development labs

Speakers

Anne Blackwell, PhD
Biocolumns Product Manager
Agilent Technologies, Inc.

Anne Blackwell is a product manager for LC columns & consumables for biomolecules at Agilent Technologies, with over a decade of experience in biomolecule analysis. Anne initially joined Agilent as an LC/MS applications scientist working on metabolomics and ion mobility workflows. She moved to the bio LC columns team as a product support scientist, focused on helping customers develop robust methods and troubleshooting challenges. Anne received her Ph.D. in analytical chemistry from the University of Arizona, where her study focused on native MS of non-covalent protein assemblies.

Matthew L. Turner, PhD
Biocolumns Product Manager
Agilent Technologies, Inc.

Matthew Turner is a product manager of Biocolumns at Agilent Technologies, Inc. supporting the cell and gene therapy market. Previously Matthew worked as a field applications scientist for four years supporting cell and gene therapy customers in their genomic and proteomic analysis and lab scale purification workflows. Matthew received his Ph.D in biochemistry and structural biology from the University of California, Davis in 2016 with a focus on structural neuronal proteins and their effect on synaptic plasticity before pursuing his career supporting biopharma customers.

Register Free: https://www.biopharminternational.com/bp_w/glycomics