Airlift Single-Use Disposable Bioreactor Systems
Cellexus has pioneered airlift single-use disposable bioreactor systems that reportedly eliminate the need for mechanical mixing. Instead, the systems use bubbles to move cells and nutrients, providing optimal aeration for rapid growth of cells. This method of gentle agitation is suitable for microbial fermentation and cell culture.
Because of the low mechanical sheer forces inherent to the system (no impellers are used), phage and lentiviral production have been early use applications/adopters. Traditional stirred-tank bioreactors use mechanical agitation to provide mixing and aeration of the culture volume. This method of agitation can generate large shear forces that can damage the health of the cells, thus reducing lentiviral vector yields.
The single-use airlift technology uses disposable bioreactor bags to eliminate cleaning and contamination, reducing downtime between runs and decreasing time to market. The system can work in volumes from 1.5 L to 50 L, with compact systems offering a comparably low footprint (1).
Spivey, C. A Look at New Airlift Technology in Single Use Bioreactor Systems. BioPharmInternational.com, Dec. 11, 2023.