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The testing of new convecdiff membranes shows this material to be well suited for lab- and commercial-scale bind-and-elute applications.
Convecdiff membranes, a new type of chromatographic material that combines high protein-binding capacity with high productivity while providing scalability, are introduced. The convecdiff membrane prototypes presented in this study reach a dynamic binding capacity of approximately 40 g/L at residence times in the seconds range while pressure drop is below 2.5 bar at a bed height of several millimeters. The robustness of the new material is proven up to 200 cycles. The material performance regarding yield, impurity reduction, and other results observed during cycling is comparable to the performance of established materials, and no performance decline occurred during cycling. With all these characteristics, the material is well suited for protein bind-and-elute applications ranging from laboratory to production scale.
Submitted: March 2, 2022
Accepted: July 21, 2022
Katrin Töppner*, Katrin.Toeppner@Sartorius.com, is Product Development (PD) scientist; Patrick Adametz*, Patrick.Adametz@Sartorius.com, is process engineer; Ricarda A. Busse is product manager for Membrane Chromatography Consumables; and Volkmar Thom is head of PD Purification Materials; all at Sartorius Stedim Biotech. Chaz Goodwine is lead downstream scientist at AGC Biologics.
*To whom all correspondence should be addressed.
Vol. 36, No. 1
When referring to this article, please cite it as Töppner, K; Adametz, P.; Goodwine, C.; Busse, R.A.; Thom, V. Complex Analytical Workflows Increasingly Require Robust Bioassays. BioPharm International 2023, 36 (1), 22–27.