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For part 2 of this article, read "Purification of Antibodies Using Novel Convecdiff Membranes Part 2: Robust Performance at Comparable Product Quality" in the Feb. 1, 2023 issue of BioPharm International.
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Purification of Antibodies Using Novel Convecdiff Membranes Part 1: Providing High Binding Capacity at Short Residence Time and Low Pressure Drops
Published on:
Katrin Töppner, Patrick Adametz, Chaz Goodwine, Ricarda A. Busse, Volkmar Thom
BioPharm International, BioPharm International, January 2023, Volume 36, Issue 01
Pages: 22–27
The testing of new convecdiff membranes shows this material to be well suited for lab- and commercial-scale bind-and-elute applications.
Convecdiff membranes, a new type of chromatographic material that combines high protein-binding capacity with high productivity while providing scalability, are introduced. The convecdiff membrane prototypes presented in this study reach a dynamic binding capacity of approximately 40 g/L at residence times in the seconds range while pressure drop is below 2.5 bar at a bed height of several millimeters. The robustness of the new material is proven up to 200 cycles. The material performance regarding yield, impurity reduction, and other results observed during cycling is comparable to the performance of established materials, and no performance decline occurred during cycling. With all these characteristics, the material is well suited for protein bind-and-elute applications ranging from laboratory to production scale.
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Peer-reviewed research
Submitted: March 2, 2022
Accepted: July 21, 2022
About the authors
Katrin Töppner*, Katrin.Toeppner@Sartorius.com, is Product Development (PD) scientist; Patrick Adametz*, Patrick.Adametz@Sartorius.com, is process engineer; Ricarda A. Busse is product manager for Membrane Chromatography Consumables; and Volkmar Thom is head of PD Purification Materials; all at Sartorius Stedim Biotech. Chaz Goodwine is lead downstream scientist at AGC Biologics.
*To whom all correspondence should be addressed.
Article details
BioPharm International
Vol. 36, No. 1
January 2023
Pages: 22–27
Citation
When referring to this article, please cite it as Töppner, K; Adametz, P.; Goodwine, C.; Busse, R.A.; Thom, V. Complex Analytical Workflows Increasingly Require Robust Bioassays. BioPharm International 2023, 36 (1), 22–27.
Download Issue: BioPharm International, January 2023
