Considerations During Development of a Protein A-Based Antibody Purification Process

January 15, 2001
Eric Cunningham

,
Harish Iyer

,
Felicia Henderson

,
James Welbb

,
Lynn Conley

,
John Hanson

,
Christopher Bork

Volume 15, Issue 1

by Harish Iyer, Felicia Henderson, Eric Cunningham, James Welbb, John Hanson, Christopher Bork, and Lynn Conley, IDEC Pharmaceuticals Corporation Scale-up changes in an antibody purification process can increase final product purity, make the process more robust, and reduce processing time. This case study focuses on the initial purification step -- protein A chromatography -- and offers data collected from several years of process development work on many different antibodies.