Storing an Ion-Exchange Chromatography Gel in Dilute Alkali During Recycling Improves Cleaning

April 15, 2002

BioPharm International, BioPharm International-04-01-2002, Volume 15, Issue 4

by Jane Turton, Central Public Health Laboratory and Zain Moola This case study shows that storing an ion-exchange chromatography gel in dilute alkalai, followed by removal of the solubized material, enhances gel cleaning. More material is removed from the gel during column recycling, and carry over of material from one cycle to the next is substantially reduced.