- BioPharm International-04-01-2003
- Volume 16
- Issue 4
Optimized Nutrient Additives for Fed-Batch Cultures
by Stephen F. Gorfien, GIBCO Cell Culture R&D, Invitrogen Corporation William Paul, David Judd, Lia Tescione, and David W. Jayme Cell density, longevity, and expression are the economics of cell cultures in biopharmaceutical production ? and rich growth media are the investment. Glucose and glutamine are primary energy sources for culture growth, but their associated metabolic wastes can actually harm cell cultures. Adapting cell lines to reduced levels of glucose and glutamine, then feeding the culture chemically defined nutrient supplements, increases cell viability, maximizes cell density, and increases product expression.
Articles in this issue
over 22 years ago
Virus Inactivation in the 1990s and into the 21st Centuryover 22 years ago
Getting Ready for Risk-Based GMPsover 22 years ago
GMP Issues: Breaking Groundover 22 years ago
Guest Editorial: Benchmarking Biotechover 22 years ago
Inside Washington: GMP Progress ReportNewsletter
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