Characterizing Vaccines with the Light Scattering Toolkit

January 5, 2021
Wyatt Technology

BioPharm International Sponsored eBooks, BioPharm International Sponsored eBooks-01-06-2021, Issue 1

This paper includes a brief introduction to light scattering instruments and techniques, and how they are coupled with fractionation techniques. For several classes of vaccines – including subunit vaccines, mRNA-LNPs, polysaccharide conjugates, and plain old viruses – case studies demonstrate the utility and breadth of analyses using the light scattering toolkit. These include measuring size distributions, characterizing protein-protein binding, and quantifying glycan content in glycoproteins or nucleic acid cargo in nanocarriers. Multiple ways to detect aggregation and other measures of stability are also described.