Standards and Best Practices to Support Trace Metal Quantitation in Cell Culture Chemically Defined Media and Hydrolysates–

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Review best practices for measurement of trace elements in cell culture media and hydrolysates, developed in a collaboration between USP and the BioPhorum Operations Group. Live: Tuesday, Mar. 3, 2020 at 11am EST | 8am PST | 4pm GMT | 5pm CET On demand available after airing until Mar. 3, 2021 Register free

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Event Overview:
Many elements such as copper, manganese, zinc, and iron have significant biological relevance, and are well established as key contributors to glycoprotein synthesis and glycosylation that can impact biologics manufacturing. The identification and controls of trace element impurities in a presumed highly pure compendium-grade individual components are essential in a global supply chain of raw materials for chemically defined media and hydrolysate formulations. In this focus on trace elements in cell-culture media, participants will learn the following:

  • Establish best practices guide for measurement of single digit low parts-per-billion measurement using inductively coupled plasma mass spectrometry (ICP-MS) and ICP-optical emission spectrometry (ICP-OES) instruments

  • Understand an approach to sample preparations and sample digestions

  • Review matrix interference removal and polyatomic interferences do’s and don’ts

Key Learning Objectives:

  • Create awareness within the industry for recognizing that trace elements are critical for biologics and can impact product quality

  • Measurement of techniques for biologics is very different from small molecule approach

  • Collaboration is essential between media manufacturer and biopharma industry to identify contaminants and establish control

  • Share information about USP and BioPhorum activities to support best practices and standards in support of upstream bioprocessing

Speakers: Dr. Bala Ramanathan, Senior Scientist, Janssen

Dr. Julian Goy, Facilitator/Account Manager, BioPhorum

Dr. Diane McCarthy, Senior Manager, Science and Standards, Biologics Pipeline Development, USP

Time and dateTuesday, Mar. 3, 2020 at 11am EST | 8am PST | 4pm GMT | 5pm CET

On demand available after airing until Mar. 3, 2021

Sponsor: USP

Register free: