
One-step Purification of 5’-DMT-on Oligonucleotides
**Wednesday, November 10, 2021 at 11am EST| 8am PST| 4pm GMT| 5pm CET** Unfold the power of oligonucleotide purification using a hydrophobic interaction resin in one-step chromatography method.
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Event Overview:
Oligonucleotide purification and analysis methods have grown to meet the demands of expanded pipelines for oligonucleotide therapies in recent years. In this study, we will demonstrate the effectiveness of a one-step hydrophobic interaction chromatography (HIC) method for the purification of 5′-DMT-on oligonucleotides. We will detail a method that is designed for capture, and direct on-column cleavage, of 5′-DMT-on oligonucleotides. Additionally, we will detail analytical methods including reversed phase chromatography, analytical anion exchange chromatography, and size exclusion chromatography, which will ensure the high purity and quality of the final product.
- An effective one-step purification of 5′-DMT-on oligonucleotides by hydrophobic interaction chromatography, with on-column DMT cleavage.
- The selected phenyl-phase chemistry resin has a dynamic binding capacity >45 mg/mL resin for 5′-DMT-on oligonucleotides.
- The on-column DMT cleavage method results in high product recovery while maintaining >95% product purity.
Key Learning Objectives:
- Simplify purification of 5′-DMT-on oligonucleotides using a one-step hydrophobic interaction chromatography method.
- Understand the power of hydrophobic interaction chromatography for 5′-DMT-on oligonucleotide purifications.
- Learn how to ensure the high purity and best quality of the final product.
Who Should Attend:
- Chromatographers, method developers and process engineers who are involved with the development and platform design for purification of oligonucleotides.
- Process Engineers
- Purification scientists
Speaker
William “Bill” Evans
Applications Scientist, Process Chromatography
Tosoh Bioscience LLC
Sponsor
Tosoh
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