Introducing Dissolved CO2 as a Critical Process Parameter in Bioprocessing

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Wednesday, June 23, 2021 at 10am EDT| 7am PDT| 3pm BST| 4pm CEST

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Event Overview:

Maintaining an optimal dissolved carbon dioxide (dCO2) level in a culture medium makes it possible to optimize productivity from batch to batch and to maintain defined quality attributes. Measuring dCO2 during cell culture is a critical process parameter and has significant benefits impacting productivity, quality, and process control. 

In this webcast, gain an understanding of dCO2 monitoring, and the control of cell culture from the bench to the large-scale manufacturing. Topics include the following:

  • What is the role of dCO2 from a metabolic point of view and its impact on cells when dCO2 is accumulating in media?
  • Why is dCO2 defined in cell culture as a critical parameter?
  • What is the benefit of monitoring and controlling dCO2 during pre-clinical and commercial phases?

Key Learning Objectives:

  • Review ways to gain better process control and transparency in dCO2 measurement in real-time.
  • Learn how to optimize productivity from batch to batch while also maintaining quality
  • Understand the benefits of monitoring and controlling dCO2 during pre-clinical and commercial phase in production.

Who Should Attend:

  • Microbiologists, Process Engineers, R&D scientists, Process Development, MTECH, MSAT, bioprocessing engineers at pilot and manufacturing scale, working upstream with fermentation or cell culture models.


Olivier R. Berteau
Global Pharmaceutical Industry Manager
Mettler-Toledo Ingold

Olivier R. Berteau, global pharmaceutical industry manager at Mettler-Toledo Ingold, started his career working for the defense industry and became interested in the design of PODs and the design of modular systems. From 1992, with the monitoring and guidance of Professor Henri Blachere, he developed bioreactor and fermenter systems. He has been involved in strategic initiatives for bioprocessing technologies including hemoglobin as an oxygen transporter for cell culture, multi-sensor (5 parameters) in a 25 mm peak sphere moving freely in a bioreactor, capacitance sensors, fully automated fermentation at mini scale (BioPOD), and acoustic single-use perfusion. In 2003, he began to take an interest in the concepts of continuous manufacturing, drawing inspiration from a 1971 patent demonstrating the performance of continuous cultures of yeast for more than 9 months without inducing cross-contamination. In 2013, before joining METTLER TOLEDO, he patented a method in cell culture using scale-out concepts under perfusion. Olivier represents mor than 25 years of hands-on operating experience within the biopharmaceutical industry with expertise in technology, technology development, and product development for upstream bioprocessing applications.

Benjamin Asher
Sr. Product Manager- Bioproduction
Thermo Fisher Scientific

Benjamin Asher is the product manager for bench-scale bioprocess, sensor technologies and automated downstream purification in Thermo Fisher Scientific’s BioProcess Division. He has a bachelor’s degree in biochemistry from Case Western Reserve University and is a member of the American Chemical Society, Society of Industrial Microbiology, and Alpha Chi Sigma Professional Chemistry Fraternity. Ben has more than 25 years of experience in bioprocessing and started his career at Amersham Life Science as a fermentation scientist. He gained valuable skills in visual analytics, data mining, and business intelligence during his career at Spotfire Inc. Ben recently joined Thermo Fisher Scientific after a 15-year career at Eppendorf where he was most recently the director of North American bioprocess sales.

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