Featured Resources for Oligonucleotides Separations
Fundamentals of Oligonucleotide Separation by HPLC
From siRNA to mRNA to DNA, separating full-length product from impurities can be challenging. Depending on the nature of the oligonucleotide you are interested in, you will be able to make informed choices about what approach to use to characterize or purify your molecule.
View the Webinar
Evaluation of New High pH Stable, Superficially-porous Particle Columns for the Reversed-phase Separation of Oligonucleotides
In this work, we evaluate the uses of new high pH stable, superficially-porous particles for oligo separations for fast and high resolution analysis, and compare them with totally porous particles.
High Performance DNA Oligonucleotide Purification Using Agilent TOP-DNA
Trityl-on purification enhances the selectivity between the full-length sequence and the truncated failures. Solid phase extraction (SPE) tubes provide a low-cost method for this type of purification. Read about how Agilent TOP-DNA cartridges make it easy to quickly obtain outstanding yields of high-purity synthetic DNA oligonucleotides.
Download the Application Note
Fast and High-Resolution Reversed-Phase Separation of Synthetic Oligonucleotides
In this application note, we demonstrate fast and high-resolution separation and identification of a number of de-protected (removal of the dimethoxytrityl group (DMT)) DNA and RNA oligonucleotides, using Agilent AdvanceBio Oligonucleotide columns.
Download the Application Note
High Resolution Separations of Oligonucleotides Using PL-SAX Strong Anion-Exchange HPLC Columns
In this application note, you can learn how to obtain fast and high-resolution separations and identifications of de-protected DNA and RNA oligonucleotides to ensure High-purity products.
Download the Application Note
Direct Analysis of In-Process Oligonucleotides Without Manual Purification
Learn about direct 2D-LC analysis of oligonucleotides in solution with high salt content and online desalting performed in the first dimension (1D), followed by IP-RPLC analysis in the second dimension (2D) in this application note.
Newsletter
Stay at the forefront of biopharmaceutical innovation—subscribe to BioPharm International for expert insights on drug development, manufacturing, compliance, and more.
eBook: Free up your plasmid purification workflows
August 2nd 2025Discover how automated plasmid purification can free up your lab's workflows and increase efficiency, scalability, and consistency without compromising quality. Get your copy of our eBook to explore real-world insights, actionable strategies, and innovative products you need to future-proof your pDNA purification processes.
Effortless maxi-scale plasmid purification
August 2nd 2025Plasmid DNA purification has a vital role in molecular biology research. pDNA isolated at maxi scale is utilized in a variety of downstream applications, including mRNA synthesis, vaccine development, antibody production, cell and gene therapy and viral vector production. Learn about this new automated process.
Accelerating Plasmid DNA Research
August 2nd 2025Watch this webinar on how to improve your plasmid DNA research using automated and high-throughput extraction methods that are now available. High yield maxi prep can be hands free, and high-throughput pDNA midi prep is possible with magnetic bead-based technology.
Beyond SaaS: How Service-as-a-Software and Agentic AI Are Powering Labs of the Future
August 2nd 2025Globally, scientific labs generate unprecedented volumes of data through cloud-based platforms, yet most of this valuable information remains fragmented and buried across different systems. While traditional software-as-a-service (SaaS) solutions have addressed the accessibility problem, labs still struggle with data overload, forcing scientists to spend countless hours searching for information that should be readily available. This whitepaper presents AI-powered SaaS, the next advancement in lab informatics that is transforming how lab workers interact with their data, enabling natural language conversations that deliver contextualized insights exactly when needed.
