Join us for this webcast, in which Zuzana Demianova will present the analysis of low abundant mannose glycopeptides of adalimumab along with a highly sensitive peptide mapping analysis of adalimumab. Live: Wednesday, Aug. 26, 2020 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST On demand available after final airing until Aug. 26, 2021
Event Overview: http://www.chromatographyonline.com/lcgc_w/ce_methods_series
Glycosylation is a common post-translational modification (PTM) that occurs during the production of therapeutic monoclonal antibodies (mAb) and many other protein therapeutics.Reversed-phase liquid chromatography coupled to mass spectrometry (RP-LC–MS) has become a primary technique for analyzing glycosylation on antibody and protein therapeutics. However, it is challenging to chromatographically resolve some types of low abundant glycoforms based on hydrophobicity, such as high mannose‑containing glycopeptides. Capillary electrophoresis with electrospray ionization (CESI) coupled to MS provides a separation mechanism orthogonal to LC–MS based approaches, including identification and relative quantification of analytes.
This presentation provides a comparison of the peptide mapping analysis of adalimumab and its PTMs performed by both CESI-MS as well as LC–MS. Approximately 95% of the sequence was mapped with high confidence and 14 various glycopeptides were confirmed on the MS and MS/MS level using diagnostic ions. CESI-MS proved to be a strong complementary technology to ensure a high level of characterization for complex PTM analysis for the example biotherapeutic.
Key Learning Objectives:
Speaker: Dr. Zuzana Demianova, Sr. BioPharma Application Scientist, SCIEX
Time and Date: Wednesday, Aug. 26, 2020 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST
On demand available after airing until Aug. 26, 2021.
Register free: http://www.chromatographyonline.com/lcgc_w/ce_methods_series