Advancing mAb Charge Variant Characterization Using Native, On-Line IEX-MS Technology

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Learn tips for efficient analyses of mAb charge variants, at both the intact and subunit level, using ion-exchange chromatography (IEX) and detection via mass spectrometry (MS). Gain insight on tools and tactics for accurate quantitation and cleaner spectra with less undesired adduct noise or super charging. Live: Wednesday, Dec. 4, 2019 11am EST | 8am PST | 4pm GMT | 5pm CST On demand available after airing until Sept. 10, 2020 Register free

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Event Overview:
Use of cation-exchange (CEX) chromatography for accurate quantitation of mAb charge variants with optical detection is a well-accepted and used technology. This technical webcast details the development and use of CEX with mass spectrometry (MS) volatile eluents to provide high quality MS data of well separated native mAb species. Learn tips and tricks on implementing this technology to address the characterization and monitoring of charge variants of mAbs from research to product commercialization. Topics discussed in this webcast will include:

  • Principles and practice using cation-exchange chromatography 

  • Capillary electrophoresis vs. ion exchange (IEX)-based separations

  • Development of a volatile IEX eluent for online MS characterization
  • How the technology can demonstrate accurate quantitation, cleaner spectra with less adduct noise or super charging, MS of native proteins, and reduced ion suppression

  • Method robustness and reproducibility

  • Simplified data interpretation

  • Time savings

Key Learning Objectives:

  • Learn how online liquid chromatography (LC)–MS analysis provides cleaner spectra and simplified data interpretation 

  • Understand eluent preparation, use, and storage 

  • Review benefits and optimization of MS detector

  • Learn how to interpret MS data of mAb charge variants

Speakers: Samantha Ippoliti, Senior Scientist, Scientific Operations Applications, Waters Corporation

Andrew Schmudlach, Senior Scientist, Chemistry Technologies R&D, Waters Corporation

Time and Date: Wednesday, Dec. 4, 2019 11am EST | 8am PST | 4pm GMT | 5pm CST

On demand available after airing until Dec. 4, 2020

Sponsor: Waters Corporation

Register free: