May 28, 2013
By:
Alex Zhu, James Martosella, Phu Duong, Agilent Technologies
Reversed-phase UHPLC/HPLC is routinely used for peptide mapping, but if the digest contains hydrophilic peptides, such as glycopeptides, valuable information can be missed. This note demonstrates peptide mapping of digested glycoprotein erythropoietin (EPO) protein using an Agilent ZORBAX Rapid Resolution High Definition 300-HILIC 1.8 ?m LC column and a TOF MS. Taking advantage of the high organic solvent system of the mobile phase for HILIC (hydrophilic interaction chromatography), the glycopeptides are effectively dissolved, retained and separated by the HILIC column. This app note includes the separation, sequence coverage and comparison of reversed-phase and HILIC data to demonstrate the utility of HILIC as an orthogonal and complementary approach to RPLC/MS for peptide analysis.
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May 28, 2013
By:
James Martosella, Phu Duong, Alex Zhu, Agilent Technologies
In this note, we describe a fast and efficient liquid chromatography-TOF mass spectrometry approach to characterize an IgG derived from CHO cells. An Agilent Poroshell 300SB-C3, 5 um superficially porous IgG1 column was used to obtain accurate glycoform masses of an intact IgG1, with a subsequent analysis of papain-digeste3d IgG1 to obtain site-specific glycosylation profile information of the Fc region for further characterization of IgG1 heterogeneity. Additionally, a subsequent mAb-glyco chip LC/MS analysis was used to build a glycan accurate mass database for additional validation of the glycans identified in the TOF mass analysis.
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May 28, 2013
By:
Ali Khaki, Katherine M. Brendza, Brian E. Schultz, Roman Sakowicz, Wyatt Technology Corporation
Promiscuous non-stoichiometric inhibition can be a serious problem when screening libraries of compounds in a high-throughput format. A rapid method of detecting such compound behavior can be achieved using a DynaPro Plate Reader to examine the light scattering properties of a dilution series of known aggregating inhibitors.
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May 28, 2013
By:
Scott Gibbard, Horst Groesser Dr.
This white paper explores how product lifecycle management (PLM) can enable biopharma companies to develop innovative products that generate real economic benefits for their organizations and health benefits for patients.
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May 28, 2013
By:
Tosoh Bioscience
TOYOPEARL GigaCap Q-650S is capable of delivering oligonucleotides of comparable purity to that seen with the TSKgel® SuperQ-5PW (20) resin and at slightly higher process yields under the same loading conditions but at lower pressures.
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May 28, 2013
By:
Ravindra Gudihal Ph.D., Agilent Technologies
A method using LC/MS is described for improving the analysis PEGylated peptides and proteins using the post-column addition of a charge stripping agent to obtain simpler mass spectra for detailed structural characterization.
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May 28, 2013
By:
Lew Brown, Fluid Imaging Technologies
This application note details how imaging particle analysis automatically characterizes fibrous particles using the actual image and shape in addition to ESD. This allows each, individual fiber to be accurately measured for the degree of curl, straightness, length, width and other parameters, and to be automatically differentiated from each other.
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May 28, 2013
By:
Leu-Fen H. Lin, PhD, Richard Bunnell, PhD
A case study reviews the challenges in reducing reconstitution time of a recombinant protein. The example may provide useful information and practical guidelines for the development of other high-concentration protein drug products.
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May 1, 2013
By:
Parker Hannifin
The clarification of batch and fed-batch bioreactor systems can pose a practical challenge to biopharmaceutical manufacturers due to high cell densities. The HarvestClearTM Filtration System is an automated single-use system designed to optimize clarification of 1L to 20L high density bioreactor outputs, while reducing hands-on time and increasing operator safety.
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