A new, very high capacity cation exchange resin* for process scale applications was developed by the researchers at Tosoh Corporation. This resin, with dynamic binding capacities approaching 145 mg/mL for IgG molecules, is the newest member of the Toyopearl product line.
Recent advances in the ability of upstream processes to manufacture biotherapeutic proteins at levels up to 10 mg/mL in the cell culture fluid have placed increasing demands on downstream unit operations. One of the potential bottlenecks in the downstream processes is the binding capacity of the chromatographic resin. If more capacity can be engineered into the resin, smaller columns, improved throughput, and less buffer consumption can be utilized. Tosoh Corporation designed a new cation exchange resin that has very high binding capacity for the purification of biotherapeutic molecules to alleviate this potential bottleneck. This new resin, Toyopearl GigaCap S-650M, has a dynamic binding capacity close to 145 mg/mL for IgG and is designed to have a long lifetime, withstand high flow rates and tolerate aggressive cleaning regimens.Experimental
Toyopearl GigaCap S-650M is an ideal cation exchange resin for the chromatographic purification of biotherapeutic products. Both large and small molecules had increased binding capacities, making it a versatile resin for all proteins. With the high binding capacities, the need for very large columns and massive buffer consumption are minimized, particularly for those proteins expressed at higher levels as a result of upstream improvements. The new Toyopearl GigaCap S-650M chromatography resin is based on the same base bead as the other Toyopearl resins and, therefore, will have similar pressure-flow and chemical stability characteristics.
*Specifically designed for packed bed use.
Authors: J. Kevin O' Donnell, PhD