Over the past decade, disposable technologies have become a reality in biotech processes. The use of disposables in research and manufacturing allows high flexibility.
The aim of cultivation in a bioreactor is to produce biomolecules using animal or plant cells, microorganisms, yeast, and insect cells.
Before production can start, the right cell line is screened out and the cultivation process must be optimized, for example, scaled-up, starting cultivation with a few milliliters and ending up with several cubic metres' cultivation volume.
For more than 40 years, typical bioreactors for cell cultivation have been made of glass or stainless steel and have been used in research and commercial production processes. This traditional bioreactor was characterized by substantial investment cost resulting from the necessity for aseptic bioprocess technology, sterilization in place (SIP), cleaning in place (CIP) and validation, all requiring sophisticated instrumentation.
Disposable bioreactors with mechanical energy input represent modern alternatives to such traditional cultivation systems for some applications in lab- and pilot-scale.
The main characteristics of the single-use bioreactor are low cost, ease of operation, time saving and high process security. These parameters shorten the time-to-market.
Disposable bioreactors can be divided into two groups:
This article aims to outline the potential and limits of the disposable bioreactor based on wave-induced agitation for biotechnology use, while considering the working principle.
The working principle
This disposable bioreactor with wave agitation can be used for batch, fed-batch and a perfusion operation mode. It is shown that even fragile cells such as animal cells (CHO, NS0, fibroblasts and hybridoma) human cells (T-Lymphocytes, HEK and Perc.6), insect cells (Sf9, High5 with baculovirus) and plant cells (hairy root culture, suspension cultures and embryo culture) can be grown in sealed bags made of polyethylene.6–8