Bio-Rad Laboratories

To maintain high purity with an untagged protein, consider protein purification workflow development using the Bio Rad NGC Chromatography System, prepacked EconoFit Columns, and Stain Free gel technology. 

Read a case study about resin screening, process development, and scale-up purification of a retrovirus-like particle which was scalable, fast, and low-cost during the clinical-grade manufacture of retrovirus vectors.   

Efficient virus recovery and impurity removal are challenges for purification of viruses such as H1N1. In a single purification step, Nuvia HP-Q AEX Resin resulted in excellent recovery and impurity removal.

Traditional sequential chromatography usually requires user manipulation which may hinder reproducibility in protein purification runs. Multidimensional chromatography improves the process without sacrificing efficacy. This study compares both purification workflows demonstrating equivalent purity.