April 30, 2013
By:
Tosoh Bioscience
TOYOPEARL GigaCap DEAE-650M, a very high capacity anion exchange resin for process scale chromatography, offers chromatographers a combination of binding capacities greater than 150 g/L while maintaining peak resolution.
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April 30, 2013
By:
Fluid Imaging Technologies
This paper explains how adjusting the gray-scale threshold value using both darker and lighter pixels reveals transparent particles that would otherwise escape detection to ensure accurate count and concentration calculations. Protein agglomerate images demonstrate how properly varying threshold documents presence of one, large particle instead of several small particles.
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April 30, 2013
By:
Wyatt Technology Corporation
Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing and quality control, it is of great importance to monitor liposome size and encapsulation. Field flow fractionation (FFF) with the concomitant use of Multi-Angle Light Scattering (MALS) and Quasi-Elastic Light Scattering (QELS, aka dynamic light scattering) is an ideal tool for such characterization.
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April 30, 2013
By:
Sonja Schneider, Agilent Technologies
Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.
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April 30, 2013
By:
Rigaku Raman Technologies, Inc.
Overcoming fluorescence interference in counterfeit pharmaceutical samples is achieved using a dispersive, dual wavelength Xantus®-2 portable Raman spectrometer, equipped with both 785 nm and 1064 nm laser excitation. The dual wavelength system affords the opportunity for selection of an appropriate excitation for rapid identification of a wide range of genuine and counterfeit pharmaceuticals.
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March 26, 2013
By:
Lew Brown, Fluid Imaging Technologies
This application note explains how counting and characterizing particles based upon their actual size and shape instead of by ESD enables protein agglomerates to be automatically differentiated from silicone droplets, air bubbles and foreign matter.
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March 26, 2013
By:
James Martosella, Phu Duong, Alex Zhu, Agilent Technologies
This application note demonstrates peptide mapping performance for profiling of a tryptic digest of recombinant human erythropoietin (rhEPO) protein as a model digest for glycopeptides profiling. Like many protein therapeutics, rhEPO exhibits a great deal of heterogeneity due to modifications that occur during manufacturing. The AdvanceBio Peptide Mapping column, with an optimized C18 coating, provides excellent peptide selectivity and resolution across a broad elution range to enhance separations of the glycopeptides fragments for fast, efficient and sensitive mass spectrometry analysis.
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March 26, 2013
By:
M.Sundaram Palaniswamy, Agilent Technologies
A simple method for the analysis of disulfide linkages in mAb was developed using an Agilent Bio-inert LC System and an Agilent ZORBAX RRHD 300 Diphenyl sub-2 μm particle column.
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February 26, 2013
By:
Xuemei He, Mark Snyder, Bio-Rad
Monoclonal antibodies are currently the most important class of therapeutic proteins. This application demonstrates that a three-step nonaffinity workflow can effectively deliver highly purified monoclonal antibodies with minimal feed conditioning.
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