Whitepapers - BioPharm International

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Whitepapers

TOYOPEARL GigaCap® DEAE-650M Preserving Resolution at Increased Protein Loads

April 30, 2013

TOYOPEARL GigaCap DEAE-650M, a very high capacity anion exchange resin for process scale chromatography, offers chromatographers a combination of binding capacities greater than 150 g/L while maintaining peak resolution.

Thresholding for Accurate Detection and Measurement of Transparent Particles such as Protein Agglomerates

April 30, 2013

This paper explains how adjusting the gray-scale threshold value using both darker and lighter pixels reveals transparent particles that would otherwise escape detection to ensure accurate count and concentration calculations. Protein agglomerate images demonstrate how properly varying threshold documents presence of one, large particle instead of several small particles.

Liposome Characterization by FFF-MALS-QELS

April 30, 2013

Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing and quality control, it is of great importance to monitor liposome size and encapsulation. Field flow fractionation (FFF) with the concomitant use of Multi-Angle Light Scattering (MALS) and Quasi-Elastic Light Scattering (QELS, aka dynamic light scattering) is an ideal tool for such characterization.

Protein Separation with pH Gradients Using Composite Buffer Systems

April 30, 2013

Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.

Dual Wavelength 785 nm and 1064 nm Excitation Portable Raman Spectrometer: Rapid Authentication of Genuine and Counterfeit Pharmaceuticals

April 30, 2013

Overcoming fluorescence interference in counterfeit pharmaceutical samples is achieved using a dispersive, dual wavelength Xantus®-2 portable Raman spectrometer, equipped with both 785 nm and 1064 nm laser excitation. The dual wavelength system affords the opportunity for selection of an appropriate excitation for rapid identification of a wide range of genuine and counterfeit pharmaceuticals.

Automatically Detect, Differentiate Agglomerated Proteins

March 26, 2013

This application note explains how counting and characterizing particles based upon their actual size and shape instead of by ESD enables protein agglomerates to be automatically differentiated from silicone droplets, air bubbles and foreign matter.

High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column

March 26, 2013

This application note demonstrates peptide mapping performance for profiling of a tryptic digest of recombinant human erythropoietin (rhEPO) protein as a model digest for glycopeptides profiling. Like many protein therapeutics, rhEPO exhibits a great deal of heterogeneity due to modifications that occur during manufacturing. The AdvanceBio Peptide Mapping column, with an optimized C18 coating, provides excellent peptide selectivity and resolution across a broad elution range to enhance separations of the glycopeptides fragments for fast, efficient and sensitive mass spectrometry analysis.

Disulfide Linkage Analysis of IgG1 using an Agilent 1260 Infinity Bio-inert LC System with an Agilent ZORBAX RRHD Diphenyl sub-2 μm Column

March 26, 2013

A simple method for the analysis of disulfide linkages in mAb was developed using an Agilent Bio-inert LC System and an Agilent ZORBAX RRHD 300 Diphenyl sub-2 μm particle column.

A Purification Strategy for Clinical-Grade Monoclonal Antibody Using Hydrophobic Cation Exchange Chromatography

February 26, 2013

Monoclonal antibodies are currently the most important class of therapeutic proteins. This application demonstrates that a three-step nonaffinity workflow can effectively deliver highly purified monoclonal antibodies with minimal feed conditioning.

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