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Introducing a Novel HipH Resin for Bispecific Antibody Purification
This event is now available on demand.
Event Overview:
Join Purolite's Field Applications Specialist, Tony Thomas, and Kayla Barry, Senior Engineer from MabPlex, as they present findings on the efficient purification of several bispecific antibodies using Purolite’s novel Protein A resin that allows for elution of antibodies at higher pH. Significant improvements in performance and productivity result in significantly increased monomer purity in comparison with traditional Protein A resins.
Tony will also be taking a deep-dive into Purolite's Jetting technology, a continuous emulsification technology by which all Praesto® chromatography resins are produced. This proprietary technology results in resins with a narrow, almost uniform particle size distribution, with excellent mass transfer properties.
Key Learning Objectives
Resin comparison study with two bispecifics, Praesto Jetted A50 HipH and a standard Protein A resin
Overview of Jetting Technology.
Process intensification, Praesto Jetted A50
Introduction to Praesto Jetted A50 HipH.
Featured Speaker
Dr. Tony Thomas
Field Application Scientist
Purolite, an Ecolab Company
Dr. Tony Thomas
is a Field Application Scientist at Purolite where he supports customers across all facets of chromatography purification leveraging advanced Jetted technology within the Purolite product portfolio to achieve customer success. In his previous role, Tony was a Chromatography Product Sales Specialist at GE Healthcare Life Science (Cytiva). During his tenure, Tony was responsible for supporting a wide variety of customers with purification related challenges across all scales of process development and manufacturing. He has worked across Canada, Central/Southeast USA, and Puerto Rico.
Prior to Cytiva, Tony worked at iBio where he led a manufacturing team and was responsible for technology transfer of filtration and chromatography unit operations. As part of his graduate research at Swansea University, Tony developed new methods for material surface characterization published in the industry leading Journal of Membrane Science. He also established a downstream purification protocol for an Fc-fusion biotherapeutic for treatment of idiopathic pulmonary fibrosis.
Tony graduated from Texas A&M University – Corpus Christi with a degree in Biomedical Science and Chemistry and Texas A&M University with his Master of Public Health degree in Epidemiology. He completed his Ph.D. in Chemical Engineering at Swansea University in Wales, UK.
Speaker’s Email:
tony.thomas@purolite.com
Speaker’s LinkedIn:
Tony Thomas
Kayla Barry
Senior Downstream Engineer
MabPlex USA
Kayla Barry
is a Senior Downstream Engineer at MabPlex USA where she is responsible for the development and transfer of methods covering a wide variety of biologic constructs.
Kayla’s contributions at MabPlex USA include purification process development and tech transfer of bispecific antibodies with intrinsic stability issues, supporting IND milestones outside of platform processing.
In her previous role at SarTec Co., she worked as a Research Scientist investigating the synthesis, scale-up, and optimization of waste grease to green fuel conversion in a supercritical water catalytic thermoreactor. She designed and conducted experiments, executed distillations, and analyzed products by GC-MS, FTIR, and acid titration.
Kayla graduated from the University of Minnesota - Twin Cities with a Bachelor’s in Bioproducts and Biosystems Engineering.
For any technical questions please contact Jordan Ramesh:
Jramesh@mjhlifesciences.com
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