Whitepapers

Apr 22, 2016
BioPharm International
Agilent provides a complete ADC DAR determination solution that includes automated affinity purification with the Agilent AssayMAP Bravo, LC/MS acquisition with the Agilent 1290 Infinity UHPLC and Agilent 6550 Q-TOF, deconvolution with Agilent MassHunter BioConfirm software, and DAR determination with the Agilent BioConfirm DAR Calculator.
Apr 22, 2016
BioPharm International
Reversed-phase LC/MS is the routine method for the analysis of mAbs at intact or fragment level, which provides accurate molecular mass. The LC column and method is critical for successful separations.
Apr 21, 2016
BioPharm International
Maurice’s CE-SDS application analyses IgG purity and heterogeneity in a fast and robust format. The streamlined workflow simplifies batch set-up and Compass for iCE automatically analyzes the data for you.
Apr 19, 2016
BioPharm International
This Application Note demonstrates the analysis of charge variants of Rituximab (both innovator and biosimilar molecules) using multiple heart-cutting 2D-LC with MS detection.
Apr 13, 2016
BioPharm International
CaPure-HA hydroxyapatite resin is ideal for the intermediate (post-protein A) purification of mAbs where aggregates and other impurities are removed from the target of interest through multiple modes of product-resin interactions. While traditionally used in a bind-and-elute mode, CaPure-HA can also be used as a flow-through process step for aggregate removal.
Mar 30, 2016
BioPharm International
By BioPharm International Editors
The reversed phase analysis of an ADC using an Agilent 1290 Infinity LC system coupled to an Agilent 6550 iFunnel Q-TOF with Agilent LC columns can be extensively used to reproducibly generate accurate DAR values on both intact and reduced ADCs with excellent performance.
Mar 29, 2016
BioPharm International
This application note describes the fast, sensitive quantification of infliximab from rat plasma using the ProteinWorks eXpress Digest Kit and Protocol. The ProteinWorks eXpress Digest Kit was successfully used to purify infliximab from a typical set of standard curve and QC samples in rat plasma. A limit of quantification of 10 ng/mL was readily achieved, while maintaining excellent linearity and single digit precision.
Mar 29, 2016
BioPharm International
This Application Note describes the analysis of antibody-drug conjugates by SEC-MS using the Agilent 1290 Infinity II LC system with an Agilent 6530 Accurate Mass Q-TOF LC/MS system.`
Mar 16, 2016
BioPharm International
Resolve the challenges of analyzing high molecular weight proteins with Simple Western. It automate all steps and removes need for transfer.Highly reproducible, small sample size, quantifiable results
Mar 14, 2016
BioPharm International
The vast majority of biotherapeutics are glycosylated. Glycans attached to the proteins play a critical role in the serum half-life, efficacy, and safety of the biotherapeutic drug. Released glycan analyses are traditionally done using either optical or MS only analytical systems, and the data interpretation can be very challenging due to a lack of integrated analytical systems. In recent years, Waters has launched a series of innovative analytical tools to address the challenges faced in N-glycan analysis. This technology brief demonstrates two fit-for-purpose glycan analysis workflows for comprehensive N-linked glycan profiling and structural elucidation within the Waters Glycan Application Solution with UNIFI.
lorem ipsum