Whitepapers

Aug 24, 2015
BioPharm International
Micro-Flow Imaging (MFI) easily detects particle size and morphology on a wide range of particle contaminants. And when you add the Bot 1 Autosampler to your MFI 5000 Series system, you’ve got the automated go-to method of choice for particle analysis today. Together, this combination lets you quickly screen for any changes in levels of particle contaminants like protein aggregates and silicone oil in your biopharmaceutical formulation.
Aug 10, 2015
BioPharm International
This year, to complement the BioAnalytical and Formulation Congress agenda, we are offering FREE webinars to enhance your knowledge of the area and create debate around key industry topics. Our latest webinar is entitled Early Stage Formulation Screening Strategies for Biologics hosted by Dr. Shahid Uddin, Head of Formulation from MedImmune. Topics covered I this webinar include de-risking candidate molecules, identifying CQAs, technologies and toolboxes and troubleshooting unexpected results.
Jul 30, 2015
BioPharm International
With the emergence of new protein therapeutics, including biosimilars, biobetters, and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation.
Jul 20, 2015
BioPharm International
This application note discusses the use of a 3 μm particle size, 30 nm pore size, size exclusion chromatography (SEC) column for monitoring folded (native) and unfolded (denatured) states of a monoclonal antibody using fluorescence detection (FLD).
Jul 20, 2015
BioPharm International
When protein sample prep was automated with the AssayMAP platform, the entire workflow had CVs of less than 5% for most of the 25 peptide monitored.
Jul 20, 2015
BioPharm International
This Application Note presents different online 2D-LC concepts for the high-resolution analysis of complex N-linked glycans in erythropoietin (EPO) using the Agilent 1290 Infinity 2D-LC Solution with fluorescence detection.
Jul 09, 2015
BioPharm International
The heterogeneous nature of glycosylation causes concerns with regard to the ability to characterise. However, using a sensible strategy and techniques focused on different aspects of glycan structure, characterisation is achievable.
May 13, 2015
BioPharm International
The aggregation of biopharmaceuticals is a complex process. For efficient analysis, it’s essential to carefully select orthogonal methods to minimize gaps in the data. This article discusses advantages and disadvantages of specific methods.
Apr 14, 2015
BioPharm Bulletin
Use of ScreenExpert RoboColumns for high-throughput study of loading conditions on HyperCel™ STAR AX and MEP HyperCel Sorbents for mAb purification in flow-through mode.
Mar 26, 2015
BioPharm International
Design of Experiments (DoE), also called experimental design, is a statistical approach to process development that has gained wide acceptance in the biopharmaceutical industry. DoE is used to reduce development costs by speeding up the design process and to optimize the parameters of a particular step in the manufacturing process. The data presented here demonstrate the optimization of the CaPure-HA elution using a DoE approach.