The Multi-Attribute Method (MAM): Advancing Biopharmaceutical Discovery, Development, and Manufacturing

Oct 03, 2018

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The multi-attribute method (MAM) for structural confirmation of protein therapeutics involves a targeted search of peptide mapping data using advanced, GMP-compliant-ready software, for pre-determined components that are indicative of numerous critical quality attributes (CQA).

Protein sample preparation for MAM demands reproducible, fast, and low artifact inducing proteolytic digestion into peptides; automatable methods are favored. Peptide separations must be rapid and consistent; detection must be sufficiently selective to allow monitoring of co-eluting modified peptides that differ in mass by less than a single mass unit.

MAM enables highly accurate relative quantification (% difference) of post-translational and process-induced modifications by comparison to a reference sample. High resolution accurate mass (HRAM) mass spectrometry (MS) delivers the required sensitivity and specificity for increased confidence in the detection and quantification of post-translational modifications. Moreover, HRAM MS also brings the capability to detect additional components in parallel; this critical additional data processing capability, called ‘new peak detection’, automatically detects and flags new chromatographic components in a sample once compared to a reference.  This allows MAM to deliver both quantification of known differences, but also gives the capability to flag new unknown impurities in the sample that are present above pre-set detection limits. Low-resolution MS techniques have lower specificity and are unable to determine some critical modifications, especially where there are no chromatographic differences in modified and unmodified peptides.

Learn how the robustness of modern HRAM MS bench-top systems, combined with simple and familiar compliance-ready chromatography data system software, makes MAM both highly practical and desirable for GMP lab use.


Key Learning Objectives:

  • Learn how peptide mapping using high resolution accurate mass (HRAM) mass spectrometry provides the necessary chromatographic and mass spectral resolution to afford confident measurement of critical quality attributes (CQAs)
  • See how Orbitrap-based HRAM MS and Chromeleon CDS delivers the necessary new peak detection to allow for flagging of untargeted differences
  • Learn how MAM can be used from discovery through to manufacturing


Who Should Attend:

  • Biopharmaceutical research, development, CMC, and QC scientists
  • Protein characterization and biologic drug development professionals responsible for quality or manufacturing controls
  • Regulatory scientists looking for greater knowledge of this promising methodology



Jonathan L. Josephs, PhD, Director, Pharma & BioPharma Customer Solutions Center, Thermo Fisher Scientific


Date and Time:

Live: Wednesday, 3 October, 2018 at 11 am EDT | 8 am PDT | 1600 BST | 1700 CEST

After the final airing of the webcast on 3 October, 2018 it will be available on demand until 3 October, 2019. 

Sponsor: Thermo Fisher Scientific

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