Downstream Processing

Sep 21, 2005
BioPharm International
By BioPharm International Editors
Is it realistic to believe that tangential flow filtration (TFF), commonly used for concentration, desalting, buffer exchange, and protein fractionation, is now a viable alternative to centrifugation for cell harvest and cell lysate clarification? A new generation of membranes expands the possibilities for TFF as an economical, efficient, high-yielding process substitute, according to this case study?s author.
Sep 15, 2005
BioPharm International
By BioPharm International Editors
Filterability trials are essential to the successful evaluation of total throughput of single filters or filter combinations. As such, they must be comparable. This case study examines the differences among various devices used and suggests necessary action required to achieve comparability of filterability test trials with 47 mm disc composites.
Sep 01, 2005
BioPharm International
By BioPharm International Editors
Pharma industry equipment utilization hovers below 40 percent, which would be an unacceptable figure in most industries.
Aug 01, 2005
BioPharm International
Misinterpreting the effluent profiles obtained during tracer measurements performed for determining packing quality can often lead to excessively large percolation velocities and exaggeration of packing problems. Highly useful and reliable information can be obtained through characterization of tracer effluent curves using the method of moments, information that could be critical for successful scale-up of chromatographic steps. This is the sixth in the "Elements of Biopharmaceutical Production" series.
Aug 01, 2005
BioPharm International
By BioPharm International Editors
Subcutaneous administration is likely to be an important factor in generating an immunogenic response.
Aug 01, 2005
BioPharm International
The concentration range of proteins in human plasma spans approximately twelve orders of magnitude, with 85 to 90% of the protein mass distributed across as few as six proteins.
Jul 01, 2005
BioPharm International
Disposable technology has been used effectively as a process solution for over 25 years and new uses and applications are constantly being developed. The key to all applications is the ability to pre-sterilize components and systems with gamma radiation and package them against contamination.
Jun 01, 2005
BioPharm International
An anionic column with modified chitosan bead matrix performs well in purifying cell culture. A pair of cationic-exchange columns shows promise in purifying S25 antibody.
Jun 01, 2005
BioPharm International
By BioPharm International Editors
In a mere 30 years of development, a total of 23 MAbs and MAb-related proteins have been approved for medical treatments.
May 01, 2005
BioPharm International
Saturated fractional factorial plans minimize the number of trials by one-half or better, which saves time and money.
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