Culture of 3D Cell Aggregates in Perfusion

Jul 11, 2018

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Three-dimensional (3D) cell aggregates are of great interest for many applications, including disease modeling, drug toxicity assessment, and manufacture of stem cell-based products. Stirred-tank bioreactors are promising culture systems for 3D cell aggregates, as they allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes. Furthermore, they can be operated in perfusion mode, which allows 3D cell aggregates to be sustained longer than in traditional batch cultures.

This webcast will review a research example for process development with the human tumor cell line H157, cultivated in stirred-tank mini bioreactors as 3D cell aggregates.


Key Learning Objectives:

  • Learn about the challenges and benefits of perfusion cultivation for stem cells and 3D cell models
  • Gain insight on the impact of impeller geometry on cell growth and aggregate formation
  • Interact with an expert in a live Q&A session



Dr. Philipp Nold, Infield Application Specialist, Eppendorf AG Bioprocess Center, Juelich, Germany


Date and Time:

Live: North America | Europe Broadcasts: Wednesday, 11 July, 2018 at 10 am EDT | 9 am CDT | 1500 BST | 1600 CEST

Asia Pacific Broadcast: Wednesday, 11 July, 2018 at 1130 IST | 1400 CST | 1500 JST

After the final airing of the webcast on 11 July, 2018 it will be available on demand until 11 July, 2019. 

Sponsor: Eppendorf

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