|
Using NMR Spectroscopy to Obtain the Higher Order Structure of Biopharmaceutical Products
|
|
|
| By
Yves Aubin
,
Christopher Jones
,
Darón I. Freedberg
|
|
|
|
Simple methods can characterize polysaccharide vaccines and recombinant cytokines at high resolution.
|
|
|
|
Rapid Assessment of Molecular Similarity between a Candidate Biosimilar and an Innovator Monoclonal Antibody Using Complementary LC–MS Methods
|
|
|
| By
Martin Gilar, PhD
,
Hongwei Xie, PhD
,
Asish Chakraborty, PhD
,
Joomi Ahn
,
Ying Qing Yu, PhD
,
Weibin Chen, PhD
,
St John Skilton, PhD
,
Jeffery R. Mazzeo, PhD
,
Deepalakshmi P. Dakshinamoorthy
|
|
|
|
Intact protein LC–MS detected a mass variance of 62 Da and peptide mapping located a difference of two amino acids.
|
|
|
|
Capturing Conformational Changes in Biotherapeutics by Hydrogen Deuterium Exchange and UHPLC–MS
|
|
|
| By
Joomi Ahn
,
St John Skilton, PhD
|
|
|
|
By providing information on the relative accessibility of locations within a protein, HDX by mass spectrometry opens new windows into the higher order structure of biomolecules.
|
|
|
|
Protein Characterization Through the Stages
|
|
|
| By
Lisa Crossley, PhD
|
|
|
|
Biomanufacturers should follow a risk-based approach to decide which methods to use to characterize their products.
|
|
|
|
Biophysical Characterization for Product Comparability
|
|
|
| By
Qin Zou, PhD
,
Yin Luo, PhD
|
|
|
|
Spectroscopic methods such as circular dichroism can detect subtle differences in higher order structure before and after changes in process and formulation.
|
|
|
