Articles by Keely Mapp - BioPharm International

ADVERTISEMENT

ADVERTISEMENT

Articles by Keely Mapp

Keely Mapp


Does Tween 20 Affect Monoclonal Antibody Separation?
October 29, 2013

Tween 20 has various beneficial properties for biological analysis, such as stabilizing proteins, solubilizing protein membranes, and preventing nonspecific binding of primary and secondary antibodies. Therefore, Tween 20 is often present in samples of monoclonal antibodies that require analysis using techniques such as SEC. This app note investigates whether the presence of Tween 20 detergent directly in the sample and in the eluent during multiple injections of an IgG sample onto an Agilent Bio SEC column affects the analysis or the columns.

ADVERTISEMENT

ADVERTISEMENT

Click here