DNA Removal from Bioprocess Purification Processes
January 30, 2013
By:
3M Purification Inc.
Therapeutic proteins such as monoclonal antibodies are produced in cell culture, where impurities can result from the host cells or substrates. Examples of these impurities are host cell protein and host cell DNA. In any purification process, it is desirable to remove these impurities as early in the process as possible. This 3M Purification Inc. application brief addresses removal of DNA using cellulosic depth filters. Depth filtration is often employed for the first purification step, separation of cell mass from therapeutic proteins.
|
Viral Reduction in Biopharmaceutical Processes
November 27, 2012
By:
3M Purification Inc.
Many biotherapeutic products are produced using mammalian cell culture techniques. Contaminating viruses can enter cell culture systems from a number sources including animal derived nutrient additives, the cell line itself or through adventitious contamination via human or animal process contact. This 3M Purification Inc. Application Brief presents the use of Zeta Plus™ VR Series filters to provide viral clearance of animal derived growth media feedstreams and of downstream process purification steps such as eluate from chromatography columns.
|
Filtration of Cell Culture Growth Media and Process buffers
October 30, 2012
By:
3M Purification Inc.
This 3M Purification Inc. Application Brief presents information related to the various filtration stages and techniques used in media feeds and process buffers, two universal additives used in biopharmaceutical processes. Media feeds and process buffers require filtration to protect against the introduction of bacteria, viruses, endotoxins and particulates that can contaminate and compromise processes. The Application brief explore the most common filtration processes used in media feed and process buffer preparation, and will outline the optimal solutions for each unit operation.
|
|
