Articles by M.Sundaram Palaniswamy

M.Sundaram Palaniswamy

Articles

Disulfide Linkage Analysis of IgG1 using an Agilent 1260 Infinity Bio-inert LC System with an Agilent ZORBAX RRHD Diphenyl sub-2 μm Column
March 26, 2013
By: M.Sundaram Palaniswamy , Agilent Technologies

A simple method for the analysis of disulfide linkages in mAb was developed using an Agilent Bio-inert LC System and an Agilent ZORBAX RRHD 300 Diphenyl sub-2 μm particle column.

Development and partial validation of a SEC method for high-resolution separation and quantification of monoclonal antibodies
February 26, 2013
By: M.Sundaram Palaniswamy , Agilent Technologies

A simple and sensitive method for separation & detection of IgG1 aggregates by SEC using Agilent Bio LC and Bio-column is described. Here we also show quantitation of IgG1.

QA/QC of monoclonal antibodies: High-resolution peptide mapping using the Agilent 1260 Infinity Bio inert Quaternary LC and Agilent 1290 Infinity LC Systems
July 31, 2012
By: M.Sundaram Palaniswamy , Agilent Technologies

This Application Note describes an application solution for peptide mapping of a monoclonal antibody using the Agilent 1260 Infinity Bio-inert Quaternary LC System and proves the reliability of the solution. The bio-inertness and corrosion resistance of the instrument coupled with simple and reproducible methods make this solution particularly suitable for the QA/QC analysis of monoclonal antibody for the biopharmaceutical industry. The power range of the 1290 Infinity LC System (pressure versus flow) overcomes the challenge of increased back pressure caused by smaller particles and longer columns.