Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.
This Application Note presents the small-scale purification of a monoclonal antibody and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
This Technical Overview shows that the Agilent Buffer Advisor software together with the Agilent 1260 Infinity Bio-inert Quaternary LC System is ideal for automated protein separation by ionic strength gradients.
With the Agilent 1260 Infinity Bio-inert Quaternary LC System, phosphate compounds can be analyzed without any issues regarding the formation of phosphate-iron complexes found with stainless steel systems.