The first PEGylated biotherapeutic, pegademase, which is a bioconjugate of the bovine derived enzyme adenosine deaminase and 5 KDa molecular weight (MW) polyethylene glycol (PEG), was introduced in 1990. Pegademase is used for the treatment of individuals with severe combined immunodeficiency disease (SCID).
In this study, we evaluate Charged Surface Hybrid (CSH) C18 column chemistry for peptide separations, expecting similar improvements in chromatographic performance since most peptides under commonly employed acidic conditions contain a positive charge. This work with peptides demonstrates that this novel stationary phase exhibits greater peak capacity, unique selectivity, and less dependence on mass spectrometry signal suppression, strong ion pairing agents in peptide analysis.
The analysis of natural and synthetic opioid drugs continues to be an important aspect of forensic toxicology. A substantial percentage of arrests and/or deaths are attributed to the misuse or abuse of narcotic pain relievers such as oxycodone and hydrocodone, as well as the illegal opiate, heroin.