Peptide mapping for protein identification and QA/QC of protein pharmaceuticals is usually done by reversed-phase chromatography. This note shows cation-exchange chromatography is an alternative method for peptide mapping.
Selective enrichment of glycans, glycopeptides, or glycoproteins significantly improves the analysis of important biomolecules. This application note describes an automated workflow combining the Thermo Scientific ProSwift ConA-1S Affinity Column with a reversed-phase HPLC analysis.
Quantify both the PEGylated product and residual PEGylation reagents in less than 20 min using a 2D-LC configuration. On-line software controlled sample preparation allowed for monitoring the degree of PEGylation.
This automated 2D workflow details a high-throughput, cost-effective method to purify and characterize MAbs via size-exclusion and ion-exchange chromatography in less than one hour, providing robust MAb analysis with minimal user intervention.