Monoclonal Antibody Analysis using Solid Core HPLC Columns
March 26, 2013
By:
Ken Cook
,
Joanna Freeke
,
Valeria Barattini
,
Thermo Fisher Scientific
We demonstrate the analysis of a monoclonal antibody (MAb) using a Thermo Scientific™ Accucore™ 150-C4 HPLC column. The analysis is fast and achieves resolution of variants in the Fab region.
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Peptide Mapping: Not Just Reversed-Phase Chromatography
November 27, 2012
By:
Dai Zhenyu
,
Xu Qun
,
Jeffrey Rohrer
,
Thermo Fisher Scientific
Peptide mapping for protein identification and QA/QC of protein pharmaceuticals is usually done by reversed-phase chromatography. This note shows cation-exchange chromatography is an alternative method for peptide mapping.
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New Method Speeds and Improves Glycoprotein/Glycopeptide Isolation
September 25, 2012
By:
Dai Zhenyu
,
Xu Qun
,
Jeffrey Rohrer
,
Thermo Fisher Scientific
Selective enrichment of glycans, glycopeptides, or glycoproteins significantly improves the analysis of important biomolecules. This application note describes an automated workflow combining the Thermo Scientific ProSwift ConA-1S Affinity Column with a reversed-phase HPLC analysis.
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Qualify and Quantify PEG and PEGylated Biopharmaceuticals
July 31, 2012
By:
Christopher Crafts
,
Bruce Bailey
,
Marc Plante
,
Ian Acworth
,
Thermo Fisher Scientific
Quantify both the PEGylated product and residual PEGylation reagents in less than 20 min using a 2D-LC configuration. On-line software controlled sample preparation allowed for monitoring the degree of PEGylation.
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